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Translocation of HrpN1‐323‐CyaA into tobacco cells. Plants were infiltrated with bacterial suspensions of the wild‐type Ea273 and T3SS‐deficient mutant, Ea273‐hrcN, harbouring pCPP1553 or pCPP1729 at OD600 = 0.4. Leaf samples were taken 7 h after infiltration and the amount of cAMP was determined. cAMP is expressed as pmol/mg of total protein. The values for each strain are the means of six samples from one experiment. The bars represent standard errors. WT, wild‐type.
