Figure 5.

The effect of compounds affecting cyclic adenosine monophosphate (cAMP) levels (caffeine) and calcium signalling on Sclerotinia sclerotiorum infection of Brassica napus leaves and expression of S. sclerotiorum necrosis and ethylene‐inducing peptide 2 gene (SsNep2). The top panels show leaves 16 h after inoculation (upper frame), as well as the lesions beneath (lower frame), for mycelia that were untreated (control) or treated with caffeine (2.5 and 20 mm), cyclosporin A (CspA; 4.9 and 9.9 nm), [ethylenebis(oxonitrilo)]tetraacetic acid (EGTA) (10 mm), lanthanum chloride (LaCl₃; 1 and 10 mm), compound 48/80 (5 µm) or compound U73122 (10 mm). The bottom panels show the effect of these compounds on new mycelial growth. A sample of the mycelial preparation used to inoculate the leaves was also placed in the centre of a minimal salts–glucose (MS‐Glu) plate and radial growth was assessed after 5 days.