Table 3.
Effect of coat protein (CP) and helper component‐proteinase (HC‐Pro) mutations on virus accumulation and aphid transmission of Soybean mosaic virus (SMV).
| SMV mutant | Virus accumulation* | Aphid transmission† | |||||
|---|---|---|---|---|---|---|---|
| Mean ± SE | P value 1 | P value 2 | Exp. 1 | Exp. 2 | Exp. 3 | P value 3 | |
| Mock | 0 | 0/10 | 0/10 | 0/10 | |||
| pSMV‐G7H | 100.0 ± 0.0 | 10/10 | 9/10 | 10/10 | |||
| pSMV‐CPG12E | 86.3 ± 10.0 | 0.30 | 0/5 | 0/5 | 0/5 | 0.001 | |
| pSMV‐CPH246A | 0 | NA | NA | NA | |||
| pSMV‐CPR249A | 75.6 ± 17.8 | 0.14 | 4/5 | 5/5 | 5/5 | 0.686 | |
| pSMV‐CPD250A | 0 | NA | NA | NA | |||
| pSMV‐CPH256A | 77.7 ± 13.4 | 0.25 | 2/5 | 2/5 | 3/5 | 0.007 | |
| pSMVdHC‐HCWT | 93.0 ± 7.9 | 0.43 | 5/5 | 5/5 | 5/5 | 0.422 | |
| pSMVdHC‐HCT310A | 75.5 ± 14.8 | 0.10 | 0.19 | 0/5 | 0/5 | 0/5 | 0.001 |
| pSMVdHC‐HCE450A | 91.2 ± 4.5 | 0.22 | 0.82 | 5/5 | 4/5 | 5/5 | 0.686 |
| pSMVdHC‐HCK452A | 94.2 ± 4.4 | 0.31 | 0.87 | 4/5 | 5/5 | 5/5 | 0.686 |
| pSMVdHC‐HCR455A | 88.5 ± 11.5 | 0.23 | 0.64 | 0/5 | 0/5 | 0/5 | 0.001 |
*Virus accumulation was quantified by real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR). Total RNA was extracted from the third trifoliolate leaves of the inoculated soybean plants at 18 days post‐infiltration (dpi). Each value is the mean compared with that of pSMV‐G7H as a percentage of the wild‐type (100%) and the standard error (SE) compiled from at least three independent experiments. P values were estimated by Student's t‐test: 1for virus accumulation of pSMV‐G7H; 2for virus accumulation of pSMVdHC‐HCWT.
†Number of plants infected over number of plants inoculated by aphid transmission. Virus infection was confirmed by RT‐PCR using SMV‐specific primers. NA, not available. P values were estimated by Student's t‐test: 3for aphid transmissibility of pSMV‐G7H.