Figure 4.
MOS induced the expression levels of MAPK genes and defence‐related genes in N. benthamiana (A, C) and O. sativa (B, D) and the phytoalexin accumulation in O. sativa (E). (A, B) Tobacco and rice were sprayed with MOS (200 μg/mL), mannose, and control. Six leaves per sample were collected after 3 h. Expression levels of MAPK genes were detected using qPCR. (C) Upper leaves of tobacco seedlings were collected 12 h after foliage application of 200 mg/L MOS on lower leaves, and transcriptional levels of defence‐related genes were performed. (D, E) Rice leaves were cut off 12 h after root irrigation with 200 mg/L MOS, and transcriptional levels of defence‐related genes were performed. Phytoalexin levels in rice were detected using LC‐MS after root‐irrigation with MOS for different periods (0,12, 24, 48, and 60 h). Seedlings soaked in sterile water were used as a control. Phytoalexin levels in rice treated with MOS for different periods were compared with that at 0 h. MA, momilactone A; PA, phytocassane A; PD, phytocassane D; PE, phytocassane E. Values represent means ± SE.