Figure 6.
MOS treatment enhance tobacco (A, B) and rice (C–E) resistance against pathogens. (A) The right‐hand sides of 6‐week old tobacco leaves were infiltrated with MOS (200 μg/mL), HrpZ (500 μg/mL), chitosan (1000 μg/mL), mannose (13 μg/mL), and ddH2O. The left‐hand sides (red circles) were then inoculated with a 7 × 7 mm hyphal plug of P. nicotianae. Disease symptoms were measured after 48 h and then decolorized in ethanol. (B) Resistance evaluation based on the diameters of the lesion spots. Inhibition rate % = (diameter of control － diameter of elicitor)/diameter of control × 100. (C, D) 45‐day‐old rice plants were root‐irrigated with MOS 24 h prior to Xanthomonas oryze pv. Oryzae inoculation. After 14 days, the lesion lengths were measured. Inhibition rate % = (lesion lengths of control － lesion lengths of elicitor)/lesion lengths of control × 100. € Quantification of Xoo growth in rice after root‐irrigation with MOS, mannose, and control. CFU, colony‐forming unit; FW, fresh weight. * indicates significant difference compared with control (P < 0.05). Each treatment contains six plants and the experiment was repeated three times.