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. 2019 Jul 10;7(13):e14157. doi: 10.14814/phy2.14157

Figure 3.

Figure 3

Effects of overexpression of WT and mutant TRPM7 on acid‐induced whole‐cell currents in HEK293T cells. (A) Detection of TRPM7 and GAPDH mRNAs by RT‐PCR. Transfection with WT, D1054A, and D1054E of TRPM7 enhanced TRPM7 mRNA expression (compared to mock) without affecting GAPDH mRNA expression. Data are representative of duplicate experiments. (B) Representative I–V relationships of cationic currents at pH 7.4, pH 6.0, and pH 4.0 under ramp clamp from −200 to +100 mV in WT‐ or D1054A‐transfected cells. (C) Mean current densities at −200 mV of mock‐, WT‐, D1054A‐, or D1054E‐transfected cells in pH 7.4, pH 6.0, and pH 4.0 solutions (n = 6–14). Each data point represents the mean ± SEM. *Significantly different (P < 0.05) from the corresponding WT‐transfected data. †Significantly different (P < 0.05) from the values at pH 7.4.