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. Author manuscript; available in PMC: 2019 Jul 19.
Published in final edited form as: Cell Rep. 2019 Jun 4;27(10):2978–2989.e5. doi: 10.1016/j.celrep.2019.05.009

Figure 5. Increased R-Loop Abundance in the hst4Δ HST3-FRB Mutant.

Figure 5.

(A) Representative genome browser view of normalized DRIP-seq reads in WT (blue) and hst4Δ HST3-FRB (green) cells. NET-seq reads shown below.

(B) Heatmap of DRIP-seq reads summed over ORFs normalized to RNase-H controls for two biological replicates. Shown are genes whose DRIP-seq signal increases ≥ 1.3-fold relative to RNase-H control and the FDR for WT versus hst4Δ HST3-FRB is ≤ 0.25.

(C) Metagene analysis of normalized DRIP-seq reads for genes whose DRIP-seq signal increases ≥ 1.3-fold relativeto RNase-H control. Genes scaled to 500 bp. Shaded area represents standard error.

(D) Cumulative distribution of mean NET-seq reads in WT and hst4 HST3-FRB cells for the genes in (C).

See also Figure S4.