Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 28;7(3):27. doi: 10.3390/antib7030027

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Evaluation of antigen binding by refolded IgGs and IgG-KIH derivatives. (A) Evaluation by ELISA of CD30 binding by T427 IgG and T427-PE38 (PE38 fused to each heavy chain) in comparison to the KIH version of T427; (B) Evaluation by ELISA of ErbB2 binding by KIH FRP5-T427-PE38 (Light chain-FRP5 + Light chain-T427 + Heavy chain-FRP5-knob+Heavy chain-T427-hole-PE38) compared to the parental IgGs; (C) Evaluation by ELISA of heavy chains heterodimer formation. The KIH T427-FRP5 PE38 IgG bound ErbB2 (binding by the FRP5-knob heavy chain) and its T427-hole-PE38 was identified by anti-PE38 antibodies.