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Evaluation of “disulfide-stabilized IgG”, anti SA “tyrosine mutants” binding to streptavidin by ELISA. Binding capability was evaluated by ELISA with SA as the coated antigen using X4 dilutions starting at 10 nM of each examined antibody; 3% skim milk was used for blocking; binding was detected using HRP-conjugated goat anti-human antibody (diluted 1:5000 in PBST). Error bars represent the standard deviation of the data.
