Table 5.
Binding kinetics of the bsAbs and their parental mAbs to CD30 and SA antigens, as determined by SPR. Surface plasmon resonance binding affinities collected from a Biacore T200, of the investigated bsAbs and their parental antibodies to CD30 and SA.
| Antibody | Binding to CD30 | Binding to SA | ||||
|---|---|---|---|---|---|---|
| Ka (1/Ms) | Kd (1/s) | KD (Nm) | Ka (M-1s-1) | Kd (1/s) | KD (nM) | |
| H | 2.04 × 105 | 2.97 × 10−3 | 14.6 | 5.26 × 104 | 1.93 × 10−3 | 36.6 |
| B | 1.67 × 105 | 1.99 × 10−3 | 11.9 | 1.29 × 105 | 2.35 × 10−3 | 18.2 |
| E | 1.34 × 105 | 1.80 × 10−3 | 13.5 | 1.28 × 105 | 2.48 × 10−3 | 19.3 |
| BYY | 2.00 × 105 | 2.29 × 10−3 | 11.5 | 4.30 × 104 | 2.65 × 10−3 | 61.7 |
| EYY | 1.21 × 105 | 1.94 × 10−3 | 16 | 2.20 × 104 | 3.18 × 10−3 | 145 |
| T427 WT | 4.69 × 105 | 4.22 × 10−4 | 0.899 | - | - | - |
| αSA WT | - | - | - | 3.68 × 105 | 1.41 × 10−4 | 0.384 |
* The constants are empirical and not mechanistic.