. 2019 Feb 10;8(1):14. doi: 10.3390/antib8010014

Table 2.

Single domain antibody recognition of the whole virus antigen standards by ELISA.

Antigen Standard	Year	Vic2b-3	Yam2a-10	Vic2a20	Vic2a-6	YamNGS#1	Yam2b-9	Vic1b-10	Yam1b-9	Yam2a-1	Yam2c-16	Vic2c-8	Yam1b-6	Yam2b-2
B/Brisbane/60/2008	2008	0.34	0.16	2.30	2.42	0.08	0.45	0.79	0.30	0.82	0.45	0.29	0.50	0.07
B/Malaysia/2506/2004	2004	0.07	0.08	3.54	1.93	0.09	0.68	1.39	0.40	1.43	0.72	0.40	0.59	0.07
B/Shangdong/7/97	1997	0.39	0.14	2.58	0.32	0.06	0.69	0.96	0.30	0.92	0.51	0.28	0.31	0.08
B/Victoria/2/87	1987	0.43	0.20	3.31	0.86	0.09	0.56	1.27	0.83	1.35	1.15	0.77	1.04	0.11
B/HongKong/8/73	1973	0.46	0.39	0.06	0.07	0.13	0.34	1.48	0.66	1.41	0.72	0.56	0.29	0.09
B/Yamagata/16/88	1988	0.83	1.76	2.41	1.07	0.09	1.59	1.85	0.98	1.79	1.01	0.87	0.71	0.12
B/Yamanashi/166/98	1994	0.74	1.08	0.11	0.07	0.07	1.70	2.16	1.34	2.41	1.35	1.24	1.70	0.14
B/Harbin/7/94	1994	0.28	0.26	0.14	0.09	0.44	0.38	0.57	0.27	0.52	0.36	0.27	0.30	0.06
B/Jiangsu/10/2003	2003	0.46	0.71	1.16	0.08	0.59	0.66	1.32	0.53	1.33	0.46	0.53	0.23	0.06
B/Florida/4/2006	2006	0.42	0.40	0.16	0.07	0.83	0.62	0.64	0.20	0.68	0.31	0.18	0.32	0.07
B/Mass’/02/2012	2012	0.53	0.82	0.79	0.05	1.00	3.03	1.82	0.94	1.96	0.85	0.84	1.57	0.10
B/Phuket/3073/2013	2013	0.58	1.22	0.15	0.06	0.11	1.01	0.94	0.55	0.96	0.45	0.52	0.32	0.08
B/Utah/9/2014	2014	0.90	1.36	0.14	0.07	0.10	0.31	1.13	0.52	1.20	0.43	0.50	0.21	0.05
A/Brisbane/10/2007	2007	0.05	0.06	0.07	0.07	0.07	0.06	0.06	0.07	0.06	0.07	0.06	0.07	0.07

B-Victoria strains ranging from 1987–2008 and B-Yamagata strains from 1988–2014. Pre-lineage split strain highlighted in red. Influenza B strains used as immunogens for the sdAb library generation are highlighted in blue. Influenza A strain control highlighted in green. Table arranged to show strains moving away from pre-lineage split in time, with most recent strains being the farthest away. Values shown are the average of two independent assays. sdAb binding was tested at a single concentration of 10 µg/mL. OD₄₅₀ ≥ 1.0, OD₄₅₀ ≥ 0.5, OD₄₅₀ ≥ 2 × negative control for each sdAb. B/Mass’/02/2012 is B/Massachusetts/02/2012.