Table 3.

Epitope classification of full-length (HA0) and head domain (HA1) using surface plasmon resonance (SPR).

Single Domain Antibody	B Yam HA1 1 B/Florida /4/2006	B Yam HA0 2 B/Florida /4/2006	B Vic HA1 1 B/Brisbane /60/2008	B Vic HA0 2 B/Brisbane /60/2008	Domain	Specificity
Vic2b-3	0.27 nM	1.57 nM	0.06 nM	0.11 nM	Head	Cross reactive
Yam2a-10	3.49 nM	6.70 nM	+ 3	3.30 nM	Head	Cross reactive
Vic2a-20	173 nM	-	0.79 nM	0.48 nM	Head	Cross reactive
Vic2a-6	-	-	0.07 nM	0.08 nM	Head	B-Vic lineage
YamNGS#1	11.8 nM	2.39 nM	-	-	Head	B-Yam lineage
Yam2b-9	-	2.13 nM	-	10.0 nM	Stem	Cross reactive
Vic1b-10	-	0.28 nM	-	1.1 nM	Stem	Cross reactive
Yam1b-9	-	0.10 nM	-	0.08 nM	Stem	Cross reactive
Yam2a-1	-	0.14 nM	-	7.40 nM	Stem	Cross reactive
Yam2c-16	-	1.46 nM	-	0.76 nM	Stem	Cross reactive
Vic2c-8	-	0.13 nM	-	0.37 nM	Stem	Cross reactive
Yam1b-6	-	5.43 nM	-	0.60 nM	Stem	Cross reactive
Yam2b-2	-	10.0 nM	-	1.36 nM	Stem	Cross reactive

¹ HA1 is purified hemagglutinin head domain (Residues Met1-Arg361 of B/Florida/04/2006 and residues Met1-Arg362 of B/Brisbane/60/2008). ² HA0 is purified full-length hemagglutinin. ³ Binding could be seen but could not be resolved using the BIAevaluation^TM software.