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. 2019 Feb 12;8(1):15. doi: 10.3390/antib8010015

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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ELISA for anti-canine CD117 fully reformatted IgG1 mAbs. (A) The ELISA plate was coated with recombinant canine CD117-hFc, then probed with the seven anti-canine CD117 IgG1 mAbs. Bound mAbs were detected using horse radish peroxidase (HRP) conjugated anti-κ and anti-λ secondary antibodies. (B) The ELISA plate was coated with recombinant canine CD117-hFc and then probed with different concentrations of anti-canine CD117 mAbs pre-mixed with 50-ng/mL recombinant canine stem cell factor (SCF)-His. The presence of SCF was detected using anti-His HRP secondary antibodies. Error bars represent standard deviations.