Figure 5.

Surface plasmon resonance (SPR) sensorgrams for the kinetic interaction between anti-canine CD117 IgG1s (analyte) and the canine CD117-hFc (ligand). The CM5 sensorchip was prepared with low-level immobilization, and single-cycle kinetics were performed at a high flow rate of 100 µL/min, allowing a 1:1 binding model to be fit to the data. A 1:1 binding model (black line) was fit to the sensorgrams using BiaEvaluation software, and the calculated kinetic and equilibrium constants were recorded in Table 2.