Table 3.
Summary of anti-canine CD117 mAb characterizations on recombinant versus native canine CD117. Here, 0–4 describes the strength of mAb–CD117 interactions in an ascending order from none (“0”) to very strong (“4”). IHC: Immunohistochemistry.
| mAb | Recombinant Canine CD117 | Native Canine CD117 | |||||
|---|---|---|---|---|---|---|---|
| Flow Cytometry | ELISA | SPR | Ligand Blocking | IHC | Flow Cytometry | Suggested Epitope Type | |
| D12-κ | +4 | +4 | +4 | +2 | √ | +1 | Linear |
| C11-κ | +4 | +3 | +4 | +2 | √ | +3 | Linear |
| H1-κ | +4 | +4 | +4 | +2 | √ | +3 | Linear |
| G4-κ | +3 | +3 | +1 | +3 | × | +1 | Conformational |
| C3-λ | +2 | +1 | 0 | +1 | √ | +3 | Linear |
| E8-λ | +2 | +2 | +1 | +1 | × | +4 | Conformational |
| E7-λ | +2 | +2 | +1 | +3 | × | +3 | Conformational |