Table 1.
Functional characteristics of 41 scFv binders converted into full-length IgG1 mAbs.
| mAb ID | Enriched? Soluble Immunized, Soluble Sorted | Enriched? Soluble Immunized, Lysate Sorted | Enriched? Cells/DNA Immunized, Soluble Sorted | Enriched? Cells/DNA Immunized, Lysate Sorted | Binds Cells? | Promega In Vitro Assay EC50 (ug/mL) (Agonist) | KD (nM) [Octet, Global Fit] |
|---|---|---|---|---|---|---|---|
| tOX40.2 | Yes | No | No | No | No | not tested | not tested |
| tOX40.4 | Yes | Yes | No | No | Yes (2 peaks) | 0.044 | 9 |
| tOX40.15 | Yes | No | No | No | Yes (1 peak) | 1.276 | 6 |
| tOX40.19 | Yes | Yes | No | No | Yes (1 peak) | 0.811 | 7.7 |
| tOX40.20 | Yes | No | No | No | No | not tested | not tested |
| tOX40.21 | Yes | Yes | No | No | Yes (1 peak) | does not agonize | 7 |
| tOX40.22 | Yes | Yes | No | No | Yes (2 peaks) | 0.087 | 7.3 |
| tOX40.23 | Yes | Yes | No | No | Yes (2 peaks) | 0.419 | 5.2 |
| tOX40.24 | Yes | Yes | No | No | Yes (2 peaks) | 0.024 | 22.9 |
| tOX40.28 | Yes | No | No | No | No | not tested | not tested |
| tOX40.31 | Yes | Yes | No | No | Yes (2 peaks) | 0.043 | 22.4 |
| tOX40.33 | Yes | No | No | No | No | not tested | not tested |
| tOX40.34 | Yes | No | No | No | No | not tested | not tested |
| tOX40.35 | Yes | No | No | No | Yes (1 peak) | 4.316 | 2.7 |
| tOX40.36 | Yes | No | No | No | No | not tested | not tested |
| tOX40.37 | Yes | Yes | No | No | Yes (2 peaks) | 0.195 | 12.5 |
| tOX40.38 | Yes | No | No | No | No | not tested | not tested |
| tOX40.39 | Yes | Yes | No | No | Yes (2 peaks) | 0.199 | 90 |
| tOX40.40 | Yes | Yes | No | No | Yes (2 peaks) | 0.426 | 151 |
| tOX40.41 | No | Yes | No | No | Yes (2 peaks) | 0.068 | 58.4 |
| tOX40.42 | No | No | Yes | No | Yes (1 peak) | does not agonize | no binding |
| tOX40.43 | No | No | Yes | No | No | not tested | not tested |
| tOX40.44 | No | No | Yes | No | No | not tested | not tested |
| tOX40.45 | No | No | Yes | No | No | not tested | not tested |
| tOX40.46 | No | No | Yes | No | No | not tested | not tested |
| tOX40.47 | No | No | Yes | No | No | not tested | not tested |
| tOX40.48 | No | No | Yes | No | No | not tested | not tested |
| tOX40.49 | No | No | Yes | No | No | not tested | not tested |
| tOX40.50 | No | No | Yes | Yes | Yes (2 peaks) | 0.091 | 29.8 |
| tOX40.51 | No | No | No | Yes | No | not tested | not tested |
| tOX40.52 | No | No | No | Yes | No | not tested | not tested |
| tOX40.54 | No | No | No | Yes | Yes (2 peaks) | 0.321 | 184 |
| tOX40.55 | No | No | No | Yes | No | not tested | not tested |
| tOX40.56 | No | No | Yes | No | No | not tested | not tested |
| tOX40.57 | No | No | Yes | No | No | not tested | not tested |
| tOX40.58 | No | No | Yes | No | No | not tested | not tested |
| tOX40.59 | No | No | Yes | No | No | not tested | not tested |
| tOX40.60 | No | No | Yes | No | No | not tested | not tested |
| tOX40.61 | No | No | No | Yes | No | not tested | not tested |
| tOX40.62 | No | No | No | Yes | No | not tested | not tested |
| tOX40.63 | No | No | No | Yes | No | not tested | not tested |