Table 5.

Measured and calculated Z values of IgG, F(ab’)₂, and Fc in pH 7.4 PBS.

ID	Subclass	IgG		F(ab’)2		Fc *
		ZDHH	Zcal	ZDHH	Zcal	ZDHH	Zcal
mAb 1	IgG1	−5.6 ± 0.1	1.8	0	−0.48	−2.8 ± 0.1	1.50
	IgG2	−7.7 ± 0.6	−4.4	0	−4.59	−6.0 ± 0.6	−0.48
	IgG4	−10.6 ± 0.5	−6.5	−4.3 ± 0.8	−2.61	−10.4 ± 0.3	−4.60
	IgG4Pro	−13 ± 0.3	−6.5	−5.05 ± 0.5	−2.61	−10.4 ± 0.3	−4.60
mAb 2	IgG1	0	5.8	0	3.5	−2.8 ± 0.1	1.50
	IgG2	−3.2 ± 0.2	−0.4	0	−0.61	−6.0 ± 0.6	−0.48
	IgG4	−7.4 ± 0.2	−2.5	0	1.38	−10.4 ± 0.3	−4.60
	IgG4Pro	−9.6 ± 0.4	−2.5	0	1.38	−10.4 ± 0.3	−4.60
mAb 3	IgG1	−5.3 ± 0.5	6.0	0	3.45	−2.8 ± 0.1	1.50
	IgG2	−6.1 ± 0.3	−0.1	0	−0.36	−6.0 ± 0.6	−0.48
	IgG4	−6.1 ± 0.2	−2.2	0	1.63	−10.4 ± 0.3	−4.60
	IgG4Pro	−10.7 ± 0.4	−2.2	0	1.63	−10.4 ± 0.3	−4.60

* The value from each subclass is identical across the mAb set because it was measured on pooled Fc samples from the three mAb digestions.