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Conjugation of G2c to photosensitizer IRDye700DX. (A left) Reducing SDS-PAGE gel showing purified G2 from Escherichia coli, G2c from Saccharomyces cerevisiae, and G2c-PS. Size-separated proteins were stained with PageBlue stain. (A right) G2c-PS and free PS in the gel shown on the left, as detected with an Odyssey scanner before the PageBlue stain. (B) Binding of G2, G2c, and G2c-PS to Met ectodomain in ELISA (representative figure, mean ± SD).
