Table 2. Monomerize and Disaggregate Aβ Before Spherulite Formation^25,60.

method 1	method 2	process
dissolve 1 mg of peptide in 1 mL of TFA (takes approximately 10 min), sonicate for 30 s on ice, freeze peptide with liquid nitrogen	N/A	solubilizes Aβ effectively and disaggregates any pre-existing aggregates
lyophilize overnight	N/A	removes TFA
dissolve in 1 mL of HFIP and leave on ice for 5–10 min	dissolve in 1 mL of HFIP and leave on ice for 5–10 min	further disrupts any aggregates
divide into aliquots and dry using a rotatory evaporator at room temperature.	divide into aliquots and dry using a rotatory evaporator at room temperature.	removes HFIP