Fig. 5.

THG detection of PFC phase-change during 2-photon excitation of a ND-Cy3 suspension. (a) A small region (44x44 µm) in a water solution of ND-Cy3 nanodroplets was subjected to continuous (0-200 seconds) excitation with 1280 nm for detecting THG signals and after 50 seconds dually excited with activating 1090 nm laser (~168mW). THG signals were observed forming over time under dual excitation (b, c) and then the THG signal disappeared once 1090 nm excitation was removed at 150 seconds (d). Imaging concluded at 200 s. Scale bar = 5 µm (e) Size distribution of the THG signals over duration of the experiment.