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. Author manuscript; available in PMC: 2019 Sep 18.
Published in final edited form as: Nat Struct Mol Biol. 2019 Mar 18;26(4):322–330. doi: 10.1038/s41594-019-0200-7

Fig 6 ∣. Validation of IGF2BP3 as an indirect m6A reader and LIN28A as an anti-reader.

Fig 6 ∣

a-b, RNA pull-down assays and western blots for (a) IGF2BP3 and (b) LIN28A, using RNA probes that contain unmodified A, m6A, and U, respectively, derived from the indicated positions in the transcripts. m6A sites are marked with a red “m”. Histograms show mean of RNA pull-down from three independent replicates. The error bars represent standard error of mean (s.e.m.).Uncropped blots are shown in Supplementary Data Set 1. c. Density plot of LIN28A binding strength (log ratio) at m6A sites in Mettl3 knockout (KO) versus wild-type mES cells. P-value is calculated by two-sided t-test. The number of transcripts is 145. d-e, Signal tracks of Nanog and Sox2 showing LIN28A binding at specific loci in Mettl3 KO and wildtype mES cells.