Fig. 3.

The effects of treatment with culture medium pre-exposed to a 50 Hz MF on cellular viability and ROS production in FL cells. The culture medium was pre-exposed by a 50 Hz MF for 1 h, then FL cells were incubated with sham (sham-treated) or 50 Hz MF pre-exposed culture medium (exposure-treated) for various durations. (A) Relative cell viabilities were examined by CCK8. (B) ROS levels were evaluated by flow cytometry using DCFH-DA. Left, representative flow cytometry histograms of DCF fluorescence of the sham-treated cells (green line), exposure-treated cells (pink line), H₂0₂-treated cells (blue line), and the blank control cells not probed with DCFH-DA (red line); right, quantitative analysis of DCF mean fluorescence intensity (MFI). Values represent mean ± SD from six independent experiments. **P < 0.01, compared with the sham group.