Fig. 4.

The effects of treatment with culture medium pre-exposed to a 50 Hz MF on expression levels of proteins in the MAPK signaling pathways in FL cells. The culture medium was pre-exposed to a 50 Hz MF for 1 h, then FL cells were incubated with sham or MF pre-exposed culture medium for 1 h. Left panel, representative western blot images show the ERK and p-ERK (A), p38 and p-p38 (B) levels in FL cells after incubation with the culture medium pre-exposed to a 50 Hz MF for various durations as indicated. Right panel, histograms showing the quantitative analysis of protein bands of western blot analysis. Values represent mean ± SD from six independent experiments.