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HeLa cells were pretreated with sucrose, filipin III, nocodazole, and cytochalasin D at 37 °C for 1 h or incubated at 4 °C for 1 h. Control was not pretreated. Then, the cellular association of rhodamine-labeled liposomes and P(NIPAAm-co-DMAPAAm) liposomes was examined in the pretreated HeLa cells following incubation at 40 or 4 °C for 30 min. Mean ± SD (n = 3); *p < 0.05 vs control and **p < 0.01 vs control.
