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Electrophoretogram of RNase A (1–124) before and after removal of the His-tag by factor Xa using 15% polyacrylamide gels. (a) Time course of the His-tag removal reaction from lα-RNase A by factor Xa. (b) Comparison of the molecular weights of four isomeric RNases after removal of the His-tag using factor Xa for 20 h.
