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Elution profiles of ion-exchange chromatography using a cation-exchange column (CM-650M) with a linear gradient of 0–1 M NaCl in the presence of 20 mM Tris–HCl (pH 7.4) at a flow rate of 1 mL/min and electrophoretograms of purified Asp-isomer-containing RNase A (1–124) using 15% polyacrylamide gel; (a) lα-RNase, (b) lβ-RNase A, (c) dα-RNase A, and (d) dβ-RNase A.
