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Western blot of an SDS-PAGE of E. coli cell pellets overexpressing the various AqpZ constructs. The same cell number (based on OD600nm) was loaded in each lane. After electrophoresis and blotting, the N-terminal oligo-histidine tag is targeted by the first antibody (Qiagen), and then the blot was developed using alkaline-phosphatase-coupled secondary antibody (Santa Cruz Biotechnology).
