Table 1. Hydrodynamic Measurements of AqpZ Mutants Using Size Exclusion Chromatography and Dynamic Light Scattering.
| mutant | size exclusion (nm3) | light scattering size (nm3) | polydispersity |
|---|---|---|---|
| C20S | 169 ± 27 (n = 4) | 267 ± 51 (n = 3) | 0.29 ± 0.05 |
| W14A | 76 ± 9 (n = 3) | 137 ± 12 (n = 4) | 0.56 ± 0.03 |
| A42F | 87 ± 9 (n = 2) | 203 ± 66 (n = 6) | 0.55 ± 0.06 |
| G144F | 214 ± 11 (n = 3) | 271 ± 8 (n = 6) | 0.31 ± 0.02 |
| I176G | 215 ± 20 (n = 4) | 282 ± 53 (n = 3) | 0.54 ± 0.08 |
| W14A–G144F | 174 ± 38 (n = 2) | 243 ± 37 (n = 3) | 0.79 ± 0.05 |
| A42F–I176G | 87 ± 8 (n = 2) | 524 ± 35 (n = 3) | 0.26 ± 0.08 |