Table 3. Oligonucleotides Used for Site-Directed Mutagenesis.
name | sequence |
---|---|
W14A | 5′-CTGAATGTTTTGGTACTTTCGCGCTTGTTTTTGGTGGCTCTGG-3′ |
A42F | 5′-CATTGGTTTTGCCGGCGTGGCGTTGTTCTTCGGTCTGACCGTTCTGACGATGG-3′ |
G144F | 5′-TTGAACTGGTATTGAGTGCATTTTTCCTGTTGGTGATCCACGG-3′ |
I176G | 5′-CCTTAACCCTGATTCACTTAGGTAGTATTCCGGTGACTAACAC-3′ |