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. 2017 Jun 28;2(6):3017–3027. doi: 10.1021/acsomega.7b00261

Table 3. Oligonucleotides Used for Site-Directed Mutagenesis.

name sequence
W14A 5′-CTGAATGTTTTGGTACTTTCGCGCTTGTTTTTGGTGGCTCTGG-3′
A42F 5′-CATTGGTTTTGCCGGCGTGGCGTTGTTCTTCGGTCTGACCGTTCTGACGATGG-3′
G144F 5′-TTGAACTGGTATTGAGTGCATTTTTCCTGTTGGTGATCCACGG-3′
I176G 5′-CCTTAACCCTGATTCACTTAGGTAGTATTCCGGTGACTAACAC-3′