Table 4. Oligonucleotides Used for Inserting Fragments of AqpZ into pKTopa.
| name | sequence |
|---|---|
| H0 | 5′-TCTAAGCTTAATGTTCAGAAAATTAGCAGCTGAATGTTTT-3′ |
| H1-1 | 3′-TAGGTACCCCTAATTCCGGGAAGCCTG-5′ |
| H1-2 | 3′-AGATCTAGATCGTTAAAATGACCACCAGAAATATGACCAA-5′ |
| H1-3 | 3′-ATGGTACCCCATAACCGTTAGAAGCAAAA-5′ |
| H1-4 | 3′-TCTTCTAGAATCGCGAATTTGTCGGTTGCG-5′ |
| H1-5 | 3′-AGATCTAGAACCGGGTTAACAGAAGTGTTAGTCAC-5′ |
| H1-6 | 3′-AGATCTAGATAATCACGCTTTTCCAGCAGGG-5′ |
a
The H0 5′-3′ oligonucleotide was used in conjunction with one of the other oligonucleotides (H1-x) to amplify the N-terminal fragment of the gene covering transmembrane helices 1 to x