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. 2019 Jul 19;14(7):e0220137. doi: 10.1371/journal.pone.0220137

Fig 6. P-body accumulation in the Dhh1 phosphorylation mutants upon glucose deprivation and α-factor treatment.

Fig 6

The dhh1 deletion mutant strain (JK400) carrying DCP2::GFP (pRP1316) was transformed with the DHH1-FLAG plasmid (pJI323), vector (pRS316), or the various mutant plasmids (pJI324-329). (A) Cultures were grown in the absence (- α) or presence (+ α) of α-factor. (B) Cultures were grown in the presence or absence of glucose. Observations were made under an Olympus BX51 fluorescence microscope using a 100 X objective, and images were processed using the MetaMorph Program software. (C) Graphs represent the quantification of P-body containing cells (%), as conducted in (A) and (B) (n = 2 replicates, > 200 cells). Values are mean ± SD. *** p < 0.005.