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. 2019 Jul 19;14(7):e0220137. doi: 10.1371/journal.pone.0220137

Table 2. Primers used in this study.

Primer name Sequence
Dhh1-F GGGCTGCAGGACCAACAAACCAATA
Dhh1-R GGGACTAGTGTGATAGTAATAAAA
Dhh1-Flag-F2 GTATCGATGGATTACAAGGATGACGACGATAAGATCTAAAGAATATCTAAGAAA
Dhh1-Flag-R2 GATCTTATCGTCGTCATCCTTGTAATCCATCGATACATACTGGGGTTGTGACTG
Dhh1-T10A F CCATCAATAATAACTTCAACGCTAATAATAACAGTAATACGG
Dhh1-T10A R CCGTATTACTGTTATTATTAGCGTTGAAGTTATTATTGATGG
Dhh1-S14A F CTTCAACACTAATAATAACGCTAATACGGATCTCGATCGG
Dhh1-S14A R CCGATCGAGATCCGTATTAGCGTTATTATTAGTGTTGAAG
Dhh1-T16A F CTAATAATAACAGTAATGCGGATCTCGATCGGGAC
Dhh1-T16A R GTCCCGATCGAGATCCGCATTACTGTTATTATTAG
Dhh1-T10E F CCATCAATAATAACTTCAACGAAAATAATAACAGTAATACGG
Dhh1-T10E R CCGTATTACTGTTATTATTTTCGTTGAAGTTATTATTGATGG
Dhh1-S14E F CTTCAACACTAATAATAACGAAAATACGGATCTCGATCGGG
Dhh1-S14E R CCCGATCGAGATCCGTATTTTCGTTATTATTAGTGTTGAAG
Dhh1-T16E F CTAATAATAACAGTAATGAGGATCTCGATCGGGAC
Dhh1-T16E R GTCCCGATCGAGATCCTCATTACTGTTATTATTAG
UACU124-Ste12 F CAGGTAAGCACTGAAGACTACTTTTATAAGTGTCCCAAGCG
UACU124-Ste12 R CGCTTGGGACACTTATAAAAGTAGTCTTCAGTGCTTACCTG
STE12-qRT F GTATCTCCTAGCGACCCTAC
STE12-qRT R AGTTTGCTGGCCAGAGTTGT
ACT1-qRT F CTGCCGGTATTGACCAAACT
ACT1-qRT R CGGACATAACGATGTTACCG
ASH1-RIP F ACGACGCTTAGAGGAGTAGA
ASH1-RIP R GTTGTCGAGTTTCATCACCA
STE12-RIP F CAGTAAAGCTACTCCGGGCGA
STE12-RIP R AGGCGGGCTCATTAACGGGC