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. 2019 Jul 19;14(7):e0219999. doi: 10.1371/journal.pone.0219999

Fig 1. Expression and characterization of purified FcγRIIIA and antibodies.

Fig 1

A, Coomassie stain of hFcγRIIIA-V158, hFcγRIIIA-F158, pFcγRIIIA, and rFcγRIIIA loaded at 2.5 μg per lane under non-reducing and reducing conditions. Molecular weight of ladder identified in kilodaltons (kDa) on left of gel. B, Coomassie stain of hIgG1, hIgG1-SD/IE, hIgG1-LA/LA/PG, pIgG1, and rIgG loaded at 2.5 μg per lane under non-reducing and reducing conditions. Molecular weight of ladder identified in kilodaltons (kDa) on left of gel. C, Molecular weight based on amino acid sequence, observed molecular weight based on Coomassie stain, and monomeric content based on analytical size exclusion chromatography (SEC). D, Binding of a titration of the Tissue Factor antigen to immobilized chimeric and parent antibodies. Reportable EC50’s and their 95% confidence intervals are listed. The antigen did not bind the isotype control antibody. Antibody preparations stored at—80°C or 37°C for 3 days were used in the assay. Filled symbols, antibodies stored at -80°C; open symbols, antibodies stored at 37°C. Circles: human 25G1; squares: rabbit 25G1; triangles: pig 25G1; upside-down triangles: isotype control.