High-layer region used in the MFMO method. The high-layer
region
used in the MFMO method contains the ligand (purple ball and stick
model) and the following amino acid residues (residue numbers: 1030–1036,
1039, 1044, 1047–1052, 1054, 1058–1065, 1068–1072,
1074–1076, and 1138–1140; blue space-filled representation).
In addition, the ligand and the amino acid residues (residue numbers:
978–980, 1029–1039, 1044–1078, 1095–1101,
1125–1127, 1136–1141, and 1155), shown by the blue and
orange space-filled representation, are allowed to move during the
MM energy minimization.