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. 2018 May 4;3(5):4932–4940. doi: 10.1021/acsomega.8b00443

Table 2. Enzyme-Catalyzed Hydrolytic Deprotection of the Methyl Ester of (2RS)-4.

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enzymea time (h)b product yields (%)c
PLE 1.0 (2RS)-5 (97), 1 (3), (2RS)-4 (ndd)
CALB 0.50 (2RS)-5 (99), 1 (ndd), (2RS)-4 (ndd)
Chirazyme L-5 CA 1.5 (2RS)-5 (17), 1 (ndd), (2RS)-4 (83)
  3.0 (2RS)-5 (29), 1 (ndd), (2RS)-4 (71)
Chirazyme L-2 CB 1.0 (2RS)-5 (99), 1 (ndd), (2RS)-4 (ndd)
a

Enzyme concentration was 1.0 mg/mL. Initial concentration of (2RS)-4 was 1.0 mM in 20 mM sodium citrate buffer (pH 5.0) containing 10% (v/v) DMSO.

b

Incubation time at 40 °C.

c

Yields based on HPLC analysis.

d

nd = not detected.