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. Author manuscript; available in PMC: 2020 Jul 18.
Published in final edited form as: Cell Chem Biol. 2019 May 16;26(7):913–925.e4. doi: 10.1016/j.chembiol.2019.04.001

Figure 3. ATF6 Activation Increases Interactions between Non-amyloidogenic FTJTO and ER Proteostasis factors.

Figure 3.

A. Distribution of unnormalized (black) and normalized (red) TMT ratios of FTALLC vs. FTJTO for proteins with significant interaction changes (n=3 biological replicates). Peptides of the λ Vc domain, which is identical for ALLC and JTO (Fig. S1A), were used to normalize the TMT signal of each individual protein against the λ Vc domain peptide signal across each TMT channel.

B. Plot showing TMT interaction ratio vs. q-value for high confidence ALLC interacting proteins that copurify with FTJTO from HEK293DAX cells following treatment with vehicle or TMP (to activate ATF6) for 16 h (n=6 biological replicates). Secretory proteins are shown in red. Full data available in Supplemental Table 3.

C. Plot comparing the interaction changes of high confidence ALLC interacting proteins with either FTALLC (n=7 biological replicates) or FTJTO (n=6 biological replicates) following stress-independent ATF6 activation. The dashed line represents least-squares linear regression. The solid lines show 95% confidence intervals