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. Author manuscript; available in PMC: 2020 Jul 18.
Published in final edited form as: Cell Chem Biol. 2019 May 16;26(7):913–925.e4. doi: 10.1016/j.chembiol.2019.04.001

Figure 4. ATF6-dependent increases in ALLC interactions correlate with ER proteostasis factor expression.

Figure 4.

A. Plot comparing mRNA level for high confidence ALLC interacting proteins (measured by RNAseq in (Plate et al., 2016); n=3 biological replicates) vs. their increased interactions with FTALLC in HEK293DAX cells following stress-independent ATF6 activation (n=7 biological replicates). The dashed line shows least-squares linear regression. The solid lines show 95% confidence intervals.

B. Plot comparing cellular protein level for high confidence ALLC interacting proteins (measured by whole cell quantitative proteomics in (Plate et al., 2016); n=3 biological replicates) vs. their increased interactions with FTALLC in HEK293DAX cells following stress-independent ATF6 activation (n=7 biological replicates). The dashed line shows least-squares linear regression. The solid lines show 95% confidence intervals.

C. Plot comparing cellular protein level for high confidence ALLC interacting proteins (measured by whole cell quantitative proteomics in (Plate et al., 2016); n=3 biological replicates) vs. their increased interactions with FTALLC in HEK293DAX cells following stress-independent XBP1s activation (n=7 biological replicates). The dashed line shows least-squares linear regression. The solid lines show 95% confidence intervals.

D. Plot comparing cellular protein level for high confidence ALLC interacting proteins (measured by whole cell quantitative proteomics in (Plate et al., 2016); n=3 biological replicates) vs. their increased interactions with FTALLC in HEK293DAX cells following stress-independent ATF6 and XBP1s co-activation (n=4 biological replicates). The dashed line shows least-squares linear regression. The solid lines show 95% confidence intervals.

E. Graph showing changes in protein levels (open symbols; n=3) or FTALLC interactions (solid bars; n=4-7) for PDIA4 in HEK293DAX cells following stress-independent XBP1s (red), ATF6 (blue), or XBP1s and ATF6 (green) activation. Error bars show SEM for the individual replicates.

F. Graph showing changes in protein levels (open symbols; n=3) or FTALLC interactions (solid bars; n=4-7) for HYOU1 in HEK293DAX cells following stress-independent XBP1s (red), ATF6 (blue), or XBP1s and ATF6 (green) activation. Error bars show SEM for the individual replicates.