Skip to main content
. Author manuscript; available in PMC: 2020 Jul 18.
Published in final edited form as: Cell Chem Biol. 2019 May 16;26(7):913–925.e4. doi: 10.1016/j.chembiol.2019.04.001

Figure 5. Overexpression of ATF6-regulated pro-folding ER proteostasis factors preferentially reduces ALLC secretion.

Figure 5.

A. Graph showing the fraction secreted of FTALLC from HEK293DAX cells overexpressing the indicated ER proteostasis factor and treated with cycloheximide (CHX) for 0 or 4 h, as measured by ELISA. Fraction secreted was quantified using the following equation: fraction secreted = [FTALLC in media at t=4 h] / [FTALLC in lysate at t = 0 h]. Error bars show SEM for n > 14 replicates across > 4 independent experiments. *p<0.05, **p<0.01, ***p<0.005 for unpaired t-tests are shown.

B. Graph showing fraction secretion for FTALLC or FTJTO from HEK293DAX cells treated with CHX for 0 or 4 h, as measured by ELISA. Fraction secreted was calculated as described in Fig. 4A. Error bars show SEM for n>9 replicates across n>3 independent experiments. ***p<0.005 for unpaired t-test is shown

C. Graph showing the normalized fraction secreted of FTALLC or FTJTO from HEK293DAX cells overexpressing the indicated ER chaperoning factor. Normalized fraction secreted was calculated by the following equation: fraction secretion in cells overexpressing a given chaperone / fraction secretion in mock-transfected cells. Fraction secreted was calculated as in Fig. 4A. Error bars show SEM for n > 9 replicates collected across > 3 independent experiments. *p<0.05, ***p<0.005 for unpaired t-tests are shown.