KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| KDEL (mouse monoclonal 10C3) | Enzo Life Sciences | Cat#ADI-SPA-827-D |
| M2 anti-FLAG (mouse monoclonal) | Sigma Aldrich | Cat#F3165 |
| Anti-α-Tubulin (mouse monoclonal B-5-1-2) | Sigma Aldrich | Cat#T5168 |
| BiP/GRP-78 (mouse monoclonal E-4) | Santa Cruz Biotechnology | Cat#sc-166490 |
| β-actin (mouse monoclonal AC-15) | Sigma Aldrich | Cat#A1978 |
| GRP94 (rabbit polyclonal N1N3) | GenTex | Cat#GTX103203 |
| HYOU1 (ORP150) (rabbit polyclonal C2C3) | GenTex | Cat#GTX102255 |
| ERdj3 (DNAJB11) (rabbit polyclonal) | ProteinTech | Cat#15484-1-AP |
| PDIA4 (ERp72) (rabbit polyclonal) | ProteinTech | Cat#14712-1-AP |
| PDIA6 (rabbit polyclonal N1N3) | GenTex | Cat#GTX121275 |
| PDIA3 (ERp57) (rabbit polyclonal G117) | CellSignaling | Cat#2881S |
| free λ LC antibody (sheep polyclonal) | Bethyl Laboratories | Cat#P80-127 |
| HRP-conjugated goat anti-human λ light chain antibody | Bethyl Laboratories | Cat#A80-116P |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| 13C6,15N4-L-Arginine | Cambridge Isotopes Laboratories | Cat#CNLM-539-H |
| Anti-FLAG M1 agarose affinity gel | Sigma Aldrich | Cat#A4596-5ML |
| Tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl) | ThermoFisher | Cat#20490 |
| Trypsin (Sequencing grade modified) | Promega | Cat#5111 |
| Iodoacetamide | Sigma Aldrich | Cat#I6125 |
| RapiGest SF Surfactant | Waters | Cat#186001861 |
| purified human Bence Jones λ light chain | Bethyl Laboratories | Cat#P80-127 |
| EasyTag EXPRESS [35S] Protein Labeling Mix | PerkinElmer | Cat#NEG772002MC |
| Critical Commercial Assays | ||
| SILAC Protein Quantification kit – DMEM | ThermoFisher | Cat#A33969 |
| TMTsixplex Isobaric Label Reagent Set | ThermoFisher | Cat#90066 |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| HEK293DAX cells | this group, Shoulders et al., 2013) | HEK293DAX |
| Experimental Models: Organisms/Strains | ||
| Oligonucleotides | ||
| Recombinant DNA | ||
| Mammalian expression plasmid (pCMVI) for FTALLC | this group, Cooley et al., 2014 | ss.FT.ALLC-pCMV1 |
| Mammalian expression plasmid (pCMVI) for FTJTO | this group, Cooley et al., 2014 | ss.FT.JTO-pCMV1 |
| Mammalian expression plasmid (pDEST40) for untagged ALLC | this group, Cooley et al., 2014 | ss.ALLC-pDEST40 |
| Entry plasmid (gateway cloning) for GRP94, GRP94.pDONR223 | Addgene | Cat #82130 |
| Mammalian expression plasmid (pDEST40) for GRP94 | this paper, Genereux et al., 2015 | GRP94.pDEST40 |
| Mammalian expression plasmid for FTBiP | this group, Genereux et al., 2015 | pFLAG.BiP.WT |
| Mammalian expression plasmid (pDEST40) for ERdj3 | this group, Genereux et al., 2015 | ss.ERdj3.pDEST40 |
| Software and Algorithms | ||
| Integrated Proteomics Pipeline Suite (IP2) | Integrated Proteomics Applications, Inc. | N/A |
| RawConverter | He et al., 2015 | N/A |
| ProLuCID (IP2 Suite) | Xu et al., 2015 | N/A |
| DTASelect (IP2 Suite) | Tabb et al., 2002 | N/A |
| Census (IP2 Suite) | Park et al., 2014 | N/A |
| Other | ||