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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Photochem Photobiol. 2019 Mar 15;95(4):1052–1059. doi: 10.1111/php.13091

Figure 4.

Figure 4.

Effects of DFO on ALA-PpIX fluorescence. Cells were incubated with ALA (1 mM) alone or in combination with DFO for 4 h. (A) PpIX fluorescence was measured with a flow cytometer and shown. (B) PpIX fluorescence intensity after ALA in combination with DFO was normalized to the fluorescence intensity after ALA treatment alone to show the percent change caused by DFO treatment. (C) PpIX fluorescence intensity of vector control and FECH-knockdown SkBr3 cells was divided by the fluorescence intensity of MCF10A cells to show the fluorescence ratio. Compared with the corresponding ALA treatment alone, DFO caused a significant decrease in the PpIX fluorescence ratio in all FECH-knockdown cell lines (Asterisks are not shown in the figure for clarity). Data are presented as mean ± SD from 3 or 4 experiments. ** P < 0.01, *** P < 0.001, compared with the corresponding ALA treatment alone.