Fig. 1.

Procedures of SAFE (simplified, amplification-free, and economically efficient process) Hi-C and traditional in situ Hi-C. a Side-by-side comparison of SAFE Hi-C and in situ Hi-C procedures. The black text shows shared steps in both methods, blue and red texts correspond to steps specific for SAFE Hi-C and in situ Hi-C, respectively. b In the preparation of both in situ Hi-C and SAFE Hi-C libraries, partially complementary adapters with a 3′ thymine (T) overhang were ligated to repaired and 3′ adenine (A)-tailed DNA fragments having been captured on streptavidin beads. The sequence of adaptors is listed in Supplementary Table 1