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. 2019 Jul 19;5:120. doi: 10.1038/s41420-019-0200-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a qPCR in triplicate and IB analysis of the expressions of 14-3-3η mRNA (top) and protein (bottom) in HuH7 and HuH7^SR cells. b HuH7^SR cells were transfected by scrambled or 14-3-3η siRNA (14-3-3η KD), while HuH7 cells were transfected by scrambled or 14-3-3η plasmid (14-3-3η OE), after then, they were treated with sorafenib. Cell viabilities were analyzed in triplicate by CCK-8 solution. c qPCR analysis in triplicate of the expressions of CD133 and EpCAM mRNAs in HuH7 and HuH7^SR cells. d HuH7^SR cells were transfected by scrambled or 14-3-3η siRNA, qRT-PCR analysis of the expressions of CD133 and EpCAM mRNAs (left); flow cytometry analysis in triplicate of the ratio of CD133⁺-EpCAM⁺ cells (right). e HuH7 cells were transfected by scrambled or 14-3-3η plasmid, qRT-PCR analysis of the expressions of CD133 and EpCAM mRNAs (top); flow cytometry analysis in triplicate of the ratio of SP cells (bottom)