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. 2019 Jul 19;5:120. doi: 10.1038/s41420-019-0200-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a IB analysis of the expressions of HIF-1α and HIF-2α in HuH7 and HuH7^SR cells. b HuH7^SR cells were transfected by scrambled or 14-3-3η siRNA, IB (left) and qRT-PCT (right) analysis of the expressions of HIF-1α or HIF-2α. c After HuH7^SR cells were transfected by scrambled or 14-3-3η siRNA, they were treated by cycloheximide for 0, 4, or 8 h. IB analysis of the expression of HIF-1α. d Computer-docking (left) and IP analysis (right) of the relationship between 14-3-3η and HIF-1α proteins in HuH7^SR cells. e After HuH7^SR cells were transfected by scrambled or 14-3-3η siRNA, they were treated by MG-132 for 2 h, IP analysis of the ubiquitination of HIF-1α. f HuH7^SR cells were transfected by scrambled or 14-3-3η siRNA, while HuH7 cells were transfected by scrambled or 14-3-3η plasmid. Immunostaining analysis of the expression and intracellular distribution of HIF-1α