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. 2019 Jul 19;9:10530. doi: 10.1038/s41598-019-46804-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Behavioral biomarkers response of Astyanax aeneus exposed and unexposed to a sublethal concentration of the pesticide ethoprophos. (A) Time spent by unexposed individuals in the dark and light compartment during the detection avoidance behavior test. (B) Time spent by exposed individuals in the dark and light compartment during the detection avoidance behavior test. (C) Total distance traveled by exposed and unexposed fish during the test. (D) Total 95% Kernel utilization distribution (KUD) area used for exposed fish in each of the test configurations. (E) Total 95% (KUD) area used for unexposed fish in each of the test configuration. (F) Reaction time of exposed and unexposed individuals in response to a simulated attack of a predator dummy. Error bars show the standard deviation of the mean.