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CRISPR guide RNAs
| Guide | Top BbSI-flanking sequence | Guide sequence | Bottom BbSI-flanking sequence | PAM |
|---|---|---|---|---|
| US1-top | CACC | 5' GAGTCCCTCAGTGTGTGAGA | – | AGG |
| US1-bottom | – | 3' TCTCACACACTGAGGGACTC | CAAA | – |
| DS1-top | CACC | 5' GGGATGACACAACGGTTTCC | – | TGG |
| DS1-bottom | – | 3' GGAAACCGTTGTGTCATCCC | CAAA | – |
| DS2-top | CACC | 5' CAATGAGTCTGTACTGGAGC | – | AGG |
| DS2-bottom | – | 3' GCTCCAGTACAGACTCATTG | CAAA | – |
US upstream FIRE, DS downstream FIRE, PAM protospacer-adjacent motif
