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. 2019 Jul 19;9:10522. doi: 10.1038/s41598-019-47023-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EV uptake assay characterization. (A) EV uptake assays performed with receptor cells preloaded with Enduren and either fixed with 4% PFA (left plot) or performed at 4 °C (right plot). Statistics: **** comparison between non-fixed (purple) and fixed cells (green). Two way Anova, Tukey’s multiple comparison test. n = 4. EV uptake assay performed at 37 °C or at 4 °C (right plot). Statistics: **** compared to positive control at 37 °C (purple). Two way Anova, Dunnett’s multiple comparison test, n = 3. (B) EV uptake assay with EVs obtained by differential ultracentrifugation. Microvesicles (MVs) or Exosomes (Exs) from DSP1-CD9/DSP2 or DSP1-CD63/DSP2 cells were used. EV uptake kinetics are represented in the left plot. Bar plot of EV uptake signal at 4 h including all negative controls is shown in the right plot. 5.58 × 10⁹ EVs of each type were used per well. Statistics: **,* comparison between EV types; ####,##,# comparison with all the controls; p values = 0.0034**, 0.0395*, < 0.0001####, 0.0019##, 0.0311#. Two way Anova, Tukey’s multiple comparison test. n = 4.