Table 2.
The following table shows the number of mice reserved for preliminary and macroscopic analysis (denoted as PRE); histology and immunohistochemistry sectioning (denoted as IHC); FITC-Dextran perfusion and light-sheet fluorescent microscopy (denoted as LSFM); and whole-mount IHC (denoted as WM-IHC).
| Timepoint (days) | 0 | 4 | 7 | 14 | 21 | 28 |
|---|---|---|---|---|---|---|
| AVL | — | PRE = 3 | — | — | PRE = 3 | PRE = 3 |
| IHC = 3 | IHC = 3 | IHC = 3 | IHC = 3 | IHC = 3 | IHC = 4 | |
| — | — | LSFM = 3 | — | — | LSFM = 3 | |
| — | — | WM-IHC = 3 | — | — | WM-IHC = 3 | |
| No AVL control |
Control chambers were placed in the contra-inguinal region of experimental mice. The number of control chambers will mirror that of AVL chambers. |
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| AVL (ligated no-flow control) | — | — | — | — | — | IHC = 3 |