Fig. 1.

Small intestinal inflammation increases the recruitment of adoptively transferred monocytes. a Representative FACS plots demonstrating frequencies of CD11b⁺Ly6C⁺ monocytes among live leukocytes in bone marrow (BM) of CX3CR1^gfp/+ mice before (left panel) and after enrichment (right panel). b Expression of CX3CR1/GFP and MHCII on purified monocytes as indicated in a. c Schematic illustration of the experimental set-up for d, e. Monocytes were transferred into untreated recipients (non-manipulated mice) or one day after small intestinal manipulation (manipulated mice). d Frequencies of adoptively transferred CD45.2⁺ donor cells in small intestinal lamina propria (siLP) one day after transfer into non-manipulated and manipulated CD45.1⁺ CCR2-deficient recipients. FACS plots are gated on live CD11b⁺ cells. e Frequencies and total numbers of CD64⁺CD11b⁺Ly6C⁺ donor monocytes recovered from siLP, colonic lamina propria (cLP), liver, spleen, and BM. Bar charts illustrate data pooled from at least three independent experiments with a total of at least five mice each. Unpaired t-test; mean ± s.d.; ns not significant; ****p < 0.0001