Fig. 7.

Single-molecule localization microscopy with tetrazine-dyes. Cos-7 cells were labeled with phalloidin-TCO and different tetrazine-dyes. a dSTORM-image of actin labeled with phalloidin-TCO and H-Tet-Cy5 in the presence of photoswitching buffer. b PALM-like super-resolution image⁸ of actin labeled with phalloidin-TCO and H-Tet-Cy5B in PBS, pH 7.4. Cy5B was reduced with 0.1% NaBH₄ for 10 min before imaging. c Actin was labeled with phalloidin-TCO and H-Tet-HMSiR, a spontaneously blinking silicon rhodamine dye⁵, and imaged in PBS, pH 7.4. Irradiation was performed in all three examples using solely 640 nm laser light at irradiation intensities of ~2 kW/cm². Scale bars, 5 µm