Table 1.
Influence of 30-min or 60-min equilibration in 0 (control), 0.5, 2.0, or 5.0 mM of AICAR on the percentage of motile spermatozoa, score of progressive motility, and percentage of membrane integrity in fresh or frozen-thawed epididymal cat spermatozoa. Values are expressed as mean ± SD (n = 10 cats)
| Metrics | Exposure time | Fresh | Control | 0.5 mM AICAR | 2.0 mM AICAR | 5.0 mM AICAR |
|---|---|---|---|---|---|---|
| Subjective motility (%) | Cooled sperm | 74.0 ± 5.4A | ||||
| 30 min | 68.0 ± 4.5A,B,a | 71.0 ± 4.2A,B,a | 68.0 ± 4.5A,B,a | 63.0 ± 5.0B,a | ||
| 60 min | 67.0 ± 9.7A,a | 65.0 ± 7.1A,B,a | 57.0 ± 3.3B,b | 46.0 ± 5.5C,b | ||
| Cryopreserved sperm | ||||||
| 59.3 ± 2.1A,a | 65.6 ± 3.2B,a | 60.2 ± 3.9A,B,a | 50.3 ± 2.4C,a | |||
| 30 min | 56.5 ± 4.2A,a | 59.7 ± 2.9A,b | 50.1 ± 4.5B,b | 40.7 ± 4.9C,b | ||
| 60 min | ||||||
| Score of progressive motility | Cooled sperm | 3.8 ± 0.4A | ||||
| 30 min | 3.9 ± 0.2A,a | 3.8 ± 0.3A,a | 3.6 ± 0.5A,a | 3.4 ± 0.5A,a | ||
| 60 min | 3.5 ± 0.4A,a | 3.5 ± 0.4A,a | 2.9 ± 0.2B,b | 3.0 ± 0.3B,b | ||
| Cryopreserved sperm | ||||||
| 3.1 ± 0.3A,B,a | 3.2 ± 0.2A,a | 3.2 ± 0.1A,a | 2.8 ± 0.3B,a | |||
| 30 min | 3.0 ± 0.2A,a | 3.1 ± 0.1A,a | 2.5 ± 0.3B,b | 2.4 ± 0.2B,b | ||
| 60 min | ||||||
| Membrane integrity (%) | Cooled sperm | 72.2 ± 3.9A | ||||
| 30 min | 66.5 ± 1.9A,a | 66.4 ± 4.7A,a | 64.5 ± 1.8A,a | 56.8 ± 9.0B,a | ||
| 60 min | 60.3 ± 2.3B,b | 60.1 ± 6.2B,b | 59.5 ± 3.7B,b | 52.9 ± 2.9C,b | ||
| Cryopreserved sperm | ||||||
| 57.7 ± 3.3A,a | 59.1 ± 5.4A,a | 56.6 ± 2.9A,a | 49.4 ± 5.6B,a | |||
| 30 min | 52.5 ± 2.8A,b | 50.2 ± 3.2A,b | 50.2 ± 1.7A,b | 44.3 ± 4.3B,a | ||
| 60 min |
Fresh sample data were only compared with cooled samples
Within the same row, values with different uppercase letters between treatment groups are statistically different (P ≤ 0.05)
Within a treatment group, values with lowercase letters between incubation times are statistically different (P ≤ 0.05)